High Precision Laser Dissection System CellSurgeon

Laser Surgery with Nanometer Precision

CellSurgeon is a laser dissection and multiphoton imaging system which enables precise, contactfree 3D-nanodissection of cells and subcellular structures. CellSurgeon can be used in living cells for studies of cell dynamics. Selective manipulation of cell organelles makes it possible to assess an impact on specific cellular processes. Furthermore, CellSurgeon is suitable for optoporation, a novel highly effective method of cell transfection. Experiments of accurate cutting in living small organisms with subsequent time-lapse recordings in 3D can easily be performed by means of CellSurgeon.

Multiphoton Imaging

CellSurgeon uses a femtosecond laser not only for manipulation but also for imaging. Whenever multiphoton imaging becomes a method of choice, CellSurgeon comes into consideration with its high penetration depth and high 3D-resolution. Visualization of numerous samples is often possible even without employing fluorescent markers, which may interfere with the processes in the cell.

3D-Dissection

For many applications though, mere visualization is sometimes not sufficient, active manipulation of cellular processes is often beneficial. The laser light of CellSurgeon is only absorbed at high intensity. This occurs only at the tight focus and allows you to operate in a very precise manner. Moreover, due to high penetration depth of near-infrared laser, CellSurgeon is the ideal tool to perform imaging plus manipulation in thick samples with only minimal phototoxic effects.

Adaptive System Configuration

CellSurgeon can be configured to your specification. The modular concept of CellSurgeon makes it adaptable to various microscopes and imaging procedures. The easy-to-operate software of CellSurgeon includes modules for 3D-dissection, confocal image acquisition, and basic image processing. One-stop provider LLS ROWIAK LaserLabSolutions offers customized solutions for different applications.

Advantages of Cell Surgeon